Hela-EGFP cell line

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Hela-EGFP Order

Catalog#

YC-A012-EGFP-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

EGFP Stable Cell Lines

cell name

Hela-EGFP

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2-1：4

Species

Human

Reporter gene

EGFP

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Accession

CVCL_C8XZ(Cellosaurus)

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Hela			STR Info (Cell bank) Cell Line:HELA
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	12	13.3		12	13.3
D7S820	8	12		8	12
D16S539	9	10		9	10
VWA	16	18		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	8	12		8	12
CSF1PO	9	10		9	10
D12S391	20	24	25.0
FGA	21	21
D2S1338	17	17
D21S11	27	28
D18S51	16	16
D8S1179	12	13
D3S1358	15	18
D6S1043	18	19
PENTAE	7	17
D19S433	13	14
PENTAD	8	15
D1S1656	12	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the Hela cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a Hela-EGFP cell line that can maintain long-term, stable and high expression.

Hela-EGFP

Catalog#

YC-A012-EGFP-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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Hela-EGFP cell line

Ubigene